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Genome editing tools - メーカー・企業と製品の一覧

Genome editing toolsの製品一覧

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Genome editing tool 'GENOME CRAFT RNA'

Genome editing tool 'GENOME CRAFT RNA'

Easily achieve genome editing! CRISPR/Cas genome editing tools.

"GENOME CRAFT RNA" is a suitable tool for achieving high-efficiency and simple knock-in/out in CRISPR/Cas genome editing technology. The CRISPR/Cas9 system, which is rapidly spreading as a genome editing technology, requires specific RNA for each target sequence. With "GENOME CRAFT RNA," you can easily achieve genome editing by simply mixing it with your Cas9 and introducing it into cells. Please make use of it to enhance the efficiency of your genome editing work. 【Features】 ○ No need for cumbersome expression vector construction or careful RNA preparation tasks [GENOME CRAFT TYPE SG] ○ Production of sgRNA through enzyme synthesis → sgRNA is synthesized via in vitro transcription reactions [GENOME CRAFT TYPE CT] ○ Production of crRNA and tracrRNA through chemical synthesis → By using chemically synthesized crRNA and tracrRNA, it can be used similarly to sgRNA For more details, please contact us or download the catalog.

  • Other physicochemical equipment

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Genome Editing Tool 'GenomeCraft - Donor DNA'

Genome Editing Tool 'GenomeCraft - Donor DNA'

We will introduce ssODN synthesis, ssDNA production, and targeting vector creation!

We would like to introduce our genome editing tool, 'GenomeCraft - Donor DNA.' In "ssODN (single-stranded oligodeoxynucleotide donor DNA) synthesis," it can be used as donor DNA to knock in sequences of up to several dozen bases, including base substitutions and loxP sites. In "ssDNA (single-stranded DNA) production," we create long single-stranded DNA that cannot be synthesized using oligodeoxynucleotide synthesis, and in "targeting vector production," we produce donor DNA for gene targeting, such as gene knockout and knock-in. Please feel free to contact us when you need our services. 【Service Details】 ■ ssODN (single-stranded oligodeoxynucleotide donor DNA) synthesis ■ ssDNA (single-stranded DNA) production ■ Targeting vector production *For more details, please refer to the PDF document or feel free to contact us.

  • Contract Analysis
  • Contract Analysis
  • Analysis Services

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[Usage Example] Genome Editing Tool 'GenomeCraft'

[Usage Example] Genome Editing Tool 'GenomeCraft'

Introducing examples of the use of double knockout and gene knock-in (in mice)!

Here are some examples of the use of the genome editing tool "GenomeCraft." In the case of "double knockout (zebrafish)," Cas9 protein was mixed with sgRNA targeting two genes or GENOME CRAFT Type CT, and injected into zebrafish embryos to observe the development. One day after fertilization, abnormalities in heart formation stained with GFP were observed, and two days later, abnormalities in eye pigment formation were noted. Additionally, there are examples of use in "in vitro digestion assay," "in vivo genome editing," and "gene knock-in (mice)." [In vitro digestion assay use case] ■ Experiment details - Cas9 protein was mixed with sgRNA or crRNA + tracrRNA, along with substrate DNA fragments, to conduct DNA cleavage experiments. ■ Results: A shift in the band was confirmed, indicating that both sgRNA and crRNA + tracrRNA exhibited good DNA cleavage activity. *For more details, please refer to the PDF materials or feel free to contact us.

  • Contract Analysis
  • Contract Analysis
  • Analysis Services

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Genome editing tool "GenomeCraft Guide RNA"

Genome editing tool "GenomeCraft Guide RNA"

We provide suitable tools that achieve high efficiency and simplicity for knock-in/knock-out!

The CRISPR/Cas9 system, which is rapidly spreading as a genome editing technology, requires specific RNA for each target sequence. With Phasmac's "GENOME CRAFT RNA," you can easily achieve genome editing by simply mixing it with your existing Cas9 and introducing it into cells. There is no need for cumbersome expression vector construction or careful RNA preparation tasks anymore. Please make use of this to improve the efficiency of your genome editing work. 【Features】 ■ Approach more targets more easily ■ No need for expression vector construction or careful RNA preparation tasks ■ Simply mix with your existing Cas9 and introduce it into cells for easy genome editing ■ Functions as guide RNA for the CRISPR/Cas system using chemically synthesized crRNA and tracrRNA *For more details, please refer to the PDF materials or feel free to contact us.

  • Other information systems

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Genome editing tool 'GenomeCraft Cas9'

Genome editing tool 'GenomeCraft Cas9'

Available for use in genome editing and in vitro DNA cleavage experiments! Offering both double-strand break and single-strand break types.

"GenomeCraft Cas9" is a Cas9 protein derived from Streptococcus pyogenes, equipped with nuclear localization signals at both the N-terminus and C-terminus. We offer two types of Cas9 proteins commonly used in genome editing via CRISPR/Cas: the double-strand cutting "Cas9 protein" and the single-strand cutting "Cas9 D10A nickase," which only cleaves the antisense strand of the target sequence (the complementary strand of PAM nGG). These can be used for genome editing through microinjection, electroporation, lipofection, and other methods, as well as for in vitro DNA cleavage experiments. 【Product Lineup】 ■ Cas9 Protein ・GE-005-S (Quantity: 100μg) ・GE-005-L (Quantity: 100μg x3) ■ Cas9 D10A Nickase ・GE-006-S (Quantity: 100μg) ・GE-006-L (Quantity: 100μg x3) *For more details, please refer to the PDF document or feel free to contact us.

  • Other information systems

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Genome editing service usage examples

Genome editing service usage examples

Examples of usage such as "in vivo genome editing" and "double knockout" are introduced.

Here are examples of the use of the genome editing tool "Genome Craft." We mixed Cas9 protein with sgRNA or crRNA + tracrRNA and substrate DNA fragments, and conducted a DNA cleavage experiment. After incubating at 37°C for one hour, we performed agarose gel electrophoresis. The shift of the bands confirmed that both sgRNA and crRNA + tracrRNA exhibited good DNA cleavage activity. In addition, there are other examples of use such as "In vivo Genome Editing," "double knockout," and "gene knock-in (mouse)." [Examples of Use] ■ In vitro digestion assay ■ In vivo Genome Editing ■ Double knockout (zebrafish) ■ Gene knock-in (mouse) *For more details, please refer to the PDF document or feel free to contact us.

  • Software (middle, driver, security, etc.)

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Fasmac Genome Craft Series

Fasmac Genome Craft Series

Free gene modification is possible! We provide suitable tools for achieving high-efficiency knock-in/knock-out.

We would like to introduce the Genome Editing Tool 'Genome Craft Series' from Fasmac Co., Ltd., which is handled by Griner Japan, Inc. Genome editing technology is a powerful tool that enables free genetic modification. We provide suitable tools for achieving high-efficiency knock-in/knock-out in CRISPR/Cas-based genome editing technology. 【Product Lineup (Partial)】 ■Guide RNA 　・Genome Craft Type CT 　・Genome Craft Type SG ■Recombinant Cas9 　・Cas9 Protein 　・Cas9 D10A Nickase *For more details, please refer to the PDF materials or feel free to contact us.

  • Analytical Equipment and Devices

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